ORIGINAL ARTICLE
Year : 2012 | Volume
: 7 | Issue : 4 | Page : 226--232
Relation of oxidant-antioxidant imbalance with disease progression in patients with asthma
Asrar Ahmad1, Mohammad Shameem3, Qayyum Husain2
1 Department of Biochemistry, Faculty of Life Sciences; Department of T.B. and Respiratory Diseases, Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh, India
2 Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, India
Correspondence Address:
Qayyum Husain
Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh-202002
India
Context: Asthma is a chronic airway disorder which is associated to the inflammatory cells. Inflammatory and immune cells generate more reactive oxygen species in patients suffering from asthma which leads to tissue injury.
Aims: To investigate the role of oxidant-antioxidant imbalance in disease progression of asthmatic patients.
Settings and Design: In this study, 130 asthmatic patients and 70 healthy controls were documented.
Methods: For this malondialdehyde level, total protein carbonyls, sulfhydryls, activity of superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx), total blood glutathione, and total antioxidant capacity (FRAP) were measured.
Statistical Analysis Used: Analysis of the data was done using unpaired student t test and one-way ANOVA analysis. P < 0.05 was considered significant.
Results: The present work showed that the systemic levels of MDA (4.19 ± 0.10 nmol/ml, P < 0.001) and protein carbonyls (1.13 ± 0.02 nmol/mg, P < 0.001) were found to be remarkably higher in asthmatic patients while protein sulfhydryls (0.55 ± 0.01 mmol/l, P < 0.05) decreased as compared to controls (2.84 ± 0.12 nmol/ml, 0.79 ± 0.02 nmol/mg and 0.60 ± 0.02 mmol/l, respectively). We also observed decrease in activities of SOD (2047 ± 50.34 U/g Hb, P < 0.05), catalase (4374 ± 67.98 U/g Hb, P < 0.01), and GPx (40.97 ± 1.05 U/g Hb, P < 0.01) in erythrocytes compared to control (2217 ± 60.11 U/g Hb, 4746 ± 89.94 U/g Hb, and 48.37 ± 2.47 U/g Hb, respectively). FRAP level (750.90 ± 21.22 ΅mol/l, P < 0.05) in plasma was decreased, whereas total blood glutathione increased (0.94 ± 0.02 mmol/l, P < 0.05) as seen in control (840.40 ± 28.39 ΅mol/l and 0.84 ± 0.04 mmol/l).
Conclusions: This work supports and describes the hypothesis that an imbalance between oxidant-antioxidant is associated to the oxidative stress which plays a significant role in severity of the disease.
How to cite this article:
Ahmad A, Shameem M, Husain Q. Relation of oxidant-antioxidant imbalance with disease progression in patients with asthma.Ann Thorac Med 2012;7:226-232
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How to cite this URL:
Ahmad A, Shameem M, Husain Q. Relation of oxidant-antioxidant imbalance with disease progression in patients with asthma. Ann Thorac Med [serial online] 2012 [cited 2023 Mar 25 ];7:226-232
Available from: https://www.thoracicmedicine.org/article.asp?issn=1817-1737;year=2012;volume=7;issue=4;spage=226;epage=232;aulast=Ahmad;type=0 |
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