Figure 6: Proposed CFTR gating mechanism. CFTR being a functional channel adapts the ATP Switch gating mechanism designed for transporters. Of note, the inbuilt latch mechanism of ABC transporters is replaced by the more complex regulatory steps in CFTR [Figure 5]. Step 1: Initiation by ATP "hydrolysable ligand" binding the NBD2 catalytic site. Step 2: Formation of closed head-to-tail NBD dimer transmits conformational changes to TMDs for pore opening. Step 3: ATP hydrolysis and dimer dissociation initiates restoration. Step 4: Pi and ADP release restores channel to its basal conformation.
i and ADP release restores channel to its basal conformation.">